Flow cytometric determination of breast tumor heterogeneity

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Flow cytometric determination of the frequency and heterogeneity of expression of human melanoma-associated antigens.

We used flow cytometry to measure the expression of human melanoma antigens on cell suspensions dissociated from metastatic masses. The objective was to study the heterogeneity between tumor samples from different patients and between different tumors excised from a single patient. Fifty-three metastases excised from 34 melanoma patients were analyzed with a panel of nine murine monoclonal anti...

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Flow Cytometric Determination of the Frequency and Heterogeneity of Expression of Human Melanoma-associated Antigens1

We used flow cytometry to measure the expression of human mela noma antigens on cell suspensions dissociated from metastatic masses. The objective was to study the heterogeneity between tumor samples from different patients and between different tumors excised from a single patient. Fifty-three métastasesexcised from 34 melanoma patients »ere analyzed with a panel of nine murine monoclonal an...

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Flow Cytometric Determination of Neutrophil CD64 (nCD64) in Children with Community Acquired Pneumonia

Background The expression of CD64 in neutrophils (nCD64) has shown utility in the diagnosis of sepsis. The aim of this study was to evaluate the diagnostic value of neutrophil CD64 in pneumonia as an early marker for infection and correlate its level with the outcome of...

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Tumor Heterogeneity in Breast Cancer

Breast cancer is a heterogeneous disease and differs greatly among different patients (intertumor heterogeneity) and even within each individual tumor (intratumor heterogeneity). Clinical and morphologic intertumor heterogeneity is reflected by staging systems and histopathologic classification of breast cancer. Heterogeneity in the expression of established prognostic and predictive biomarkers...

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Flow Cytometric Determination of Estrogen Receptors in Intact Cells1

Flow cytometry has been used to detect estrogen receptor (ER) ¡nintact cells, using 1-{/v>fluoresceinyl estrone thiosemicarbazone (17FE), a ligand shown to have sufficient affinity and specificity to identify high-affinity receptors. For each concentra tion of 17FE, two fluorescent distributions were obtained: (a) "total" fluorescence due to 17FE alone; and (o) diethylstilbestrol-inhibited flu...

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ژورنال

عنوان ژورنال: Cancer

سال: 1991

ISSN: 0008-543X,1097-0142

DOI: 10.1002/1097-0142(19910301)67:5<1401::aid-cncr2820670521>3.0.co;2-4